mandegar et (Addgene inc)
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mandegar et
Mandegar Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+ckb/pgRNA-CKB+(Plasmid+%2373501)/pmc12714712-139-4-9
Average 93 stars, based on 14 article reviews
Mandegar Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+ckb/pgRNA-CKB+(Plasmid+%2373501)/pmc12714712-139-4-9
Average 93 stars, based on 14 article reviews
mandegar et - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms. Article Snippet: .. Dox was added together with fresh media. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498; RRID:Addgene_73498). Article Title: Spatially organized cellular communities form the developing human heart Article Snippet: .. To clone the PB- TNNT2:NLS-mKATE2-T2A-BsdR , we used the PB plasmid pcsj532 (a gift from K. Willert, UCSD) and used Gibson assembly (SGI, GA1200) to clone in a synthesized TNNT2 promoter (Integrated DNA Technologies), PCR-amplified NLS-mKATE2-T2A-BsdR (with polyA) from Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with Article Title: The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms Article Snippet: .. Dox was added together with fresh media. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). Article Title: RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Article Snippet: pAAVS1-NDi-CRISPRi (Gen2) , Mandegar et al. , Addgene plasmid # 73498. .. Article Title: CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition Article Snippet: dCas9-KRAB-IRES-BFP , , Addgene plasmid #85449. .. Synthesized:Article Title: Spatially organized cellular communities form the developing human heart Article Snippet: .. To clone the PB- TNNT2:NLS-mKATE2-T2A-BsdR , we used the PB plasmid pcsj532 (a gift from K. Willert, UCSD) and used Gibson assembly (SGI, GA1200) to clone in a synthesized TNNT2 promoter (Integrated DNA Technologies), PCR-amplified NLS-mKATE2-T2A-BsdR (with polyA) from Polymerase Chain Reaction:Article Title: Spatially organized cellular communities form the developing human heart Article Snippet: .. To clone the PB- TNNT2:NLS-mKATE2-T2A-BsdR , we used the PB plasmid pcsj532 (a gift from K. Willert, UCSD) and used Gibson assembly (SGI, GA1200) to clone in a synthesized TNNT2 promoter (Integrated DNA Technologies), PCR-amplified NLS-mKATE2-T2A-BsdR (with polyA) from Article Title: Parallel functional assessment of m 6 A sites in human endodermal differentiation with base editor screens Article Snippet: U6-sgRNA fragment free FNLS-ABE7.10(AW) expression plasmid (Lenti-FNLS-ABE7.10(AW)-P2A-Puro) was generated by ligating the fragment of Kpn I/ EcoR I-digested Lenti-FNLS-ABE7.10(AW)-P2A-Puro-U6-sgRNA using T4 DNA Ligase (New England Biolabs, M0202). .. LentiGuide-BSD-dTomato was generated by combining the PCR-amplified blasticidin S deaminase segment from Article Title: Parallel functional assessment of m 6 A sites in human endodermal differentiation with base editor screens. Article Snippet: U6-sgRNA fragment free FNLS-ABE7.10(AW) expression plasmid (Lenti-FNLS-ABE7.10(AW)-P2A-Puro) was generated by ligating the fragment of Kpn I/EcoR I-digested Lenti-FNLS-ABE7.10(AW)-P2A-Puro-U6-sgRNA using T4 DNA Ligase (New England Biolabs, M0202). .. LentiGuide-BSD-dTomato was generated by combining the PCR-amplified blasticidin S deaminase segment from Generated:Article Title: Parallel functional assessment of m 6 A sites in human endodermal differentiation with base editor screens Article Snippet: U6-sgRNA fragment free FNLS-ABE7.10(AW) expression plasmid (Lenti-FNLS-ABE7.10(AW)-P2A-Puro) was generated by ligating the fragment of Kpn I/ EcoR I-digested Lenti-FNLS-ABE7.10(AW)-P2A-Puro-U6-sgRNA using T4 DNA Ligase (New England Biolabs, M0202). .. LentiGuide-BSD-dTomato was generated by combining the PCR-amplified blasticidin S deaminase segment from Article Title: Parallel functional assessment of m 6 A sites in human endodermal differentiation with base editor screens. Article Snippet: U6-sgRNA fragment free FNLS-ABE7.10(AW) expression plasmid (Lenti-FNLS-ABE7.10(AW)-P2A-Puro) was generated by ligating the fragment of Kpn I/EcoR I-digested Lenti-FNLS-ABE7.10(AW)-P2A-Puro-U6-sgRNA using T4 DNA Ligase (New England Biolabs, M0202). .. LentiGuide-BSD-dTomato was generated by combining the PCR-amplified blasticidin S deaminase segment from Stable Transfection:Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with Construct:Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with Clone Assay:Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with |